人抗膜聯(lián)蛋白V檢測(cè)試劑盒

Human Anti Annexin V IgG ELISA kit

cat#3300-240-AVA

Intended Use

Human Anti-Annexin V IgG kit is for Quantitative Determination of IgGclass autoantibodies to anti- Annexin V in human serum or plasma.

General Information

Anti phospholipid syndrome (APS, Hughes Syndrome) is a systemic autoimmunedisease that causes thromboses, recurrent miscarriage, and intrauterine foetaldeath. Clinical symptoms are accompanied by the occurrence of specificautoantibodies that are detectable in the blood of patients with APS. Theseantibodies bind to phospholipids like cardiolipin, or phospholipid-binding proteinslike beta-2-glycoprotein I. The clinical symptoms of APS alone are not sufficientlyspecific to make a definitive diagnosis. Laboratory tests thus play an importantrole in the diagnosis of the disease.The diagnosis of APS is considered as confirmed when at least one clinical andone of the laboratory criteria are fulfilled. In primary APS autoantibodies againstphospholipids appear independently, while in secondary APS phospholipidantibodies are detected in conjunction with other autoimmune diseases, such aslupus erythematosus, rheumatoid arthritis, or Sj?gren’s syndrome. Phospholipidantibodies are detectable in only 1-5 % of healthy individuals, but they are foundin 16-35 % of lupus patients.

Sensitivity:Functional sensitivity was determined to be 1 U/ml.

Parallelism

In dilution experiments sera with high antibody concentrations were diluted withsample buffer and assayed in the Annexin V kit. The assay showed linearity overthe full measuring range.

Measuring range:

The calculation range of this ELISA assay is 0 - 100 U/ml

Interfering substances

No interference has been observed with haemolytic (up to 1000 mg/dl) or lipemic(up to 3 g/dl triglycerides) sera or plasma, or bilirubin (up to 40 mg/dl) containingsera or plasma. Nor have any interfering effects been observed with the use ofanticoagulants (Citrate, EDTA, Heparine). However for practical reasons it isrecommended that grossly hemolyzed or lipemic samples should be avoided.

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