Anti-dsDNA Antibody (Anti dsDNA IgM)

ELISA KIT Cat. No. 3105

PRINCIPLE OF THE TEST

Anti-ds-DNA IgM ELISA kit is based on binding of ds-DNA antibody from serum samples to native ds-DNA (recombinant) immobilized on microtiter wells. After a washing step, goat anti-human IgM-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of ds-DNA IgM present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450 nm and the concentration of anti-DNA IgM in samples is calculated on the basis of the absorbance of the negative, positive, and, calibrator standards..